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谷胱甘肽S转移酶π1(GSTp)活性蛋白

Active Glutathione S Transferase Pi (GSTp)

GSTP1; GST-P1; DFN7; FAEES3; GST3

  • 谷胱甘肽S转移酶π1(GSTp)活性蛋白 产品包装(模拟)
  • 谷胱甘肽S转移酶π1(GSTp)活性蛋白 产品包装(模拟)
  • APB090Hu01.jpg SDS-PAGE图
  • 谷胱甘肽S转移酶π1(GSTp)活性蛋白 Figure. Western Blot; Sample: Recombinant GSTp, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTp and CDK5 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保?#21183;?#20869;,在?#23454;?#30340;条件下存储,损失?#23454;?#20110;5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB090Hu01 谷胱甘肽S转移酶π1(GSTp)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB090Hu01 谷胱甘肽S转移酶π1(GSTp)活性蛋白 Cell?culture;?Activity?Assays.
PAB090Hu01 谷胱甘肽S转移酶π1(GSTp)多克隆抗体 WB; IHC; ICC; IP.
LAB090Hu81 谷胱甘肽S转移酶π1(GSTp)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB090Hu71 谷胱甘肽S转移酶π1(GSTp)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB090Hu22 谷胱甘肽S转移酶π1(GSTp)单克隆抗体 WB; IHC; ICC; IP.
LAB090Hu72 谷胱甘肽S转移酶π1(GSTp)单克隆抗体(生物素标记) WB; IHC; ICC.
LAB090Hu82 谷胱甘肽S转移酶π1(GSTp)单克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
WEB090Hu 谷胱甘肽S转移酶π1(GSTp)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB090Hu 谷胱甘肽S转移酶π1(GSTp)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB090Hu01 谷胱甘肽S转移酶π1(GSTp)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动?#31181;?#20316;ELISA试剂盒的主要材料
PGB090Hu01 谷胱甘肽S转移酶π1(GSTp)引物对 PCR

参考文献

杂志 参考文献
International Journal of Medical Sciences Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117]
Mol Neurobiol Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019]
Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007]
Neuroscience?Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218]
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